Biochemistry PhD student at Stanford University. Interested in natural products engineering and synthetic biology.

Dec
14
awarded Yearling
Dec
14
awarded Yearling
Oct
30
answered Is this a valid definition of independent assortment?
Oct
27
awarded Nice Question
Sep
30
awarded Explainer
Sep
17
awarded Nice Answer
Aug
21
accepted E coli cotransformation efficiency
Jul
29
revised E coli cotransformation efficiency
make the question more prone to get detailed answer on efficiency of co-transformation, and not so much on explanation on how to co-transform (by double selection)
Jul
29
comment E coli cotransformation efficiency
Thanks, Chris, but I was more wondering how high with efficiency (number of clones per ug DNA) you can get with co-transformation. For example for single electroporation the efficiency is around 1 × 10^10 cfu/μg so I would like to know what is the limit and whether it can be improved for co-transformation.
Jul
12
awarded Autobiographer
Jul
12
awarded Supporter
Jul
10
comment Efficiency of plasmid DNA isolation from frozen E. coli cell cultures
Thanks Chris. I tried it with 25ml cultures and I got great yields! I did not run them on a gel to check the quality of the DNA, but the sequencing results looked good!
Jul
10
accepted Efficiency of plasmid DNA isolation from frozen E. coli cell cultures
Jul
8
asked Efficiency of plasmid DNA isolation from frozen E. coli cell cultures
Jul
2
awarded Curious
Jun
2
answered What is the definition of a stringent/relaxed plasmid?
Apr
9
awarded Taxonomist
Apr
1
answered Is a sequential double transformation acceptable?
Mar
28
asked E coli cotransformation efficiency
Mar
28
asked Does yeast grow on LB agar plates?
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