Biochemistry PhD student at Stanford University. Interested in natural products engineering and synthetic biology.

Feb
19
awarded Supporter
Feb
19
awarded Autobiographer
Jan
30
awarded Notable Question
Dec
14
awarded Yearling
Dec
14
awarded Yearling
Oct
30
answered Is this a valid definition of independent assortment?
Oct
27
awarded Nice Question
Sep
30
awarded Explainer
Sep
17
awarded Nice Answer
Aug
21
accepted E coli cotransformation efficiency
Jul
29
revised E coli cotransformation efficiency
make the question more prone to get detailed answer on efficiency of co-transformation, and not so much on explanation on how to co-transform (by double selection)
Jul
29
comment E coli cotransformation efficiency
Thanks, Chris, but I was more wondering how high with efficiency (number of clones per ug DNA) you can get with co-transformation. For example for single electroporation the efficiency is around 1 × 10^10 cfu/μg so I would like to know what is the limit and whether it can be improved for co-transformation.
Jul
12
awarded Autobiographer
Jul
12
awarded Supporter
Jul
10
comment Efficiency of plasmid DNA isolation from frozen E. coli cell cultures
Thanks Chris. I tried it with 25ml cultures and I got great yields! I did not run them on a gel to check the quality of the DNA, but the sequencing results looked good!
Jul
10
accepted Efficiency of plasmid DNA isolation from frozen E. coli cell cultures
Jul
8
asked Efficiency of plasmid DNA isolation from frozen E. coli cell cultures
Jul
2
awarded Curious
Jun
2
answered What is the definition of a stringent/relaxed plasmid?
Apr
9
awarded Taxonomist
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