Jul
2
awarded Curious
Mar
1
awarded Good Question
Dec
22
awarded Yearling
Dec
22
awarded Yearling
Dec
2
awarded Popular Question
Sep
21
awarded Custodian
Sep
11
revised How to prevent protein precipitation?
edited tags
Sep
2
revised Making penicillin using animals - specifically, a goat
corrected title
Aug
27
comment Do twins "run in the family"?
I am still catching up on this topic but there are (atleast) 3 documented towns with greater than average rate of twinning: Cândido Godói, Kodinhi and Igbo-Ora. A published study on the Brazilian town might also be of help. It might be informative to look at familial twinning in these examples as beginning clues.
Aug
27
awarded Enthusiast
Aug
23
awarded Nice Question
Aug
21
revised How to find ion/water channel related genes
edited tags
Aug
21
revised How to find ion/water channel related genes
edited tags
Aug
21
comment What makes a wet dog so stinky?
The "after rain" smell is technically called "petrichor". In summary, compounds from plants emitted during dry periods get absorbed into rocks. Rain releases these compounds and the smell derives from these compounds. The wikipedia page is a good place to read more about petrichor.
Aug
20
comment Why is GTP gamma S non hydrolyzable?
I'm almost certain its got something to do with the amino acid in the P-loop that contacts the gamma phosphate, however without a reference I am not going to post it as an answer yet. I can ask my PI but then its more fun to find it out myself ;)
Aug
20
asked Why is GTP gamma S non hydrolyzable?
Aug
20
accepted Is the EC50 of an activating protein for an enzyme a good indicator for the binding affinity Kd?
Aug
20
comment Is the EC50 of an activating protein for an enzyme a good indicator for the binding affinity Kd?
bobthejoe, just thought I'd say this: You've laid out really well why interpreting Km is not always trivial. Exactly why I chose to focus on EC50 (with the trivial(?) assumption that substrate far exceeds enzyme concentration). I'm going to accept your answer, given the richness of detail that you've laid out!
Aug
16
comment Is the EC50 of an activating protein for an enzyme a good indicator for the binding affinity Kd?
bobthejoe: I like the detail in your answer, especially the hGH example is very surprising. However, my question pertains to comparing affinities and EC50 measurements, both being derived from in vitro assays with purified proteins. I would think the avidity argument might hold, especially when the same system were compared in a vesicular environment versus detergent solution.
Aug
16
revised Why do rod cells not recover after bleaching upon exposure to bright light?
Improved title
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